Prion PrPsc, mAb 1.5D7 Antibody

SKU: orb1108772

Description

Mouse monoclonal antibody to Prion PrPSC

Research Area

Immunology & Inflammation

Images & Validation

• Tested Applications: IA, IHC-Fr, PS, WB
• Dilution Range: For immunohistochemistry, and Western blotting, dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50.
• Application Notes:
  W: A reduced sample treatment and SDS-Page was used. The band size is 30 kDa (Ref.1). IHC-P: Paraffin sections were deparaffinized, rehydrated and endogenous peroxidase was quenched using 0.3% H2O2 in methanol for 20 min. As positive control BSE infected brain tissue was used and as negative control non-diseased brain tissue (Ref.1).

Key Properties

• Clonality: Monoclonal
• Isotype: Mouse IgG2b
• Immunogen: BoPrP153-165 (GSDYEDRYYRENM)
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: 1 ml (100 µg/ml) 0.2 µm filtered antibody solution in PBS, containing 0.1% bovine serum albumin and 0.02% sodium azide.
• Buffer/Preservatives: 0.2 ml (100 µg/ml) 0.2 µm filtered antibody solution in PBS, containing 0.1% bovine serum albumin and 0.02% sodium azide.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Spongiform encephalopathies