PFDN1 Antibody

SKU: orb1247073

Description

PFDN1 Antibody

Research Area

Cancer Biology

Images & Validation

• Tested Applications: ELISA, WB
• Reactivity: Human
• Predicted Reactivity: Bovine, Canine, Mouse, Rat
• Application Notes:
  Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 14kDa band observed in lysates of cell line K562 (calculated MW of 14.2kDa according to NP_002613.2). Recommended concentration: 1-3µg/mL. An additional band of unknown identity was also consistently observed at 37kDa. This band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any such results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?

Key Properties

• Antibody Type: Primary Antibody
• Host: Goat
• Clonality: Polyclonal
• Immunogen: The immunogen for this antibody is: C-DNIREMLMARRAQ
• Target: PFDN1
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Concentration: 500 ug/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

PFDN1, PDF, PFD1, prefoldin 1, prefoldin subunit 1

orb1247073 (1 ug/ml) staining of K562 lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Quick Database Links

Gene Symbol

PFDN1

UniProt

O60925

RefSeq (Protein):NP_002613.2