PPP2R1A Antibody

SKU: orb1247915

Description

PPP2R1A Antibody

Images & Validation

• Tested Applications: ELISA, WB
• Reactivity: Human
• Predicted Reactivity: Bovine, Canine, Mouse, Porcine, Rat
• Application Notes:
  Peptide ELISA: antibody detection limit dilution 1:4000.Western Blot:Approx 60-65kDa band observed in Human Thymus and Human Lymph node lysates (calculated MW of 65.3kDa according to NP_055040.2). Recommended concentration: 2-6ug/ml.

Key Properties

• Antibody Type: Primary Antibody
• Clonality: Polyclonal
• Immunogen: The immunogen for this antibody is: C-KYFAQEALTVLSLA
• Target: PPP2R1A
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Concentration: 500 ug/mL
• Disclaimer: For research use only

Alternative Names

PPP2R1A, MGC786, protein phosphatase 2 (formerly 2A), regulatory subunit A (PR 65), alpha isoform, PP2A, subunit A, R1-alpha isoform, PP2A, subunit A, PR65-alpha isoform, medium tumor antigen-associated 61 KDA protein, Serine/threonine protein phosphatase 2A, 65 KDA regulatory subunit A, alpha isoform, protein phosphatase 2 (formerly 2A), regulatory subunit A, alpha isoform, PR65A, alpha isoform of regulatory subunit A, protein phosphatase 2, medium tumor antigen-associated 61 KDA protein, protein phosphatase 2 (formerly 2A), regulatory subunit A (PR 65), alpha isoform

orb1247915 (2 ug/ml) staining of Human Thymus (A) and Human Lymph node (B) lysates (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

orb1247915 (3.75 ug/ml) staining of paraffin embedded Human Thyroid Gland. Steamed antigen retrieval with citrate buffer pH 6, AP-staining. This data is from a previous batch, not on sale.

orb1247915 (3.75 ug/ml) staining of paraffin embedded Human Heart. Steamed antigen retrieval with citrate buffer pH 6, AP-staining. This data is from a previous batch, not on sale.

Quick Database Links

Gene Symbol

PPP2R1A

UniProt

P30153

RefSeq (Protein):NP_055040.2