POLR2H Antibody

• Catalog Number: orb1260470
• Category: Antibodies
• Description: POLR2H Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, IP, WB
• Reactivity: Human, Mouse
• Isotype: IgG
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-150 of human POLR2H (NP_006223.2).
• Concentration: batch dependent
• Dilution range: WB: 1:500 - 1:2000IF: 1:50 - 1:200IP: 1:50 - 1:200
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Observed: 17kDa
• Target: POLR2H
• UniProt ID: P52434
• Storage: Store at -20°C. Avoid freeze / thaw cycles.
• Buffer/Preservatives: PBS with 0.02% sodium azide, 50% glycerol, pH 7.3.
• Alternative names: POLR2H, RPABC3, RPB17, RPB8, hsRPB8
• Note: For research use only
• Application notes: WB: 1:500 - 1:2000IF: 1:50 - 1:200IP: 1:50 - 1:200
• Expiration Date: 12 months from date of receipt.

Western blot analysis of extracts of various cell lines, using POLR2H antibody (orb1260470) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Immunofluorescence analysis of U2OS cells using POLR2H antibody (orb1260470). Blue: DAPI for nuclear staining.

Immunoprecipitation analysis of 200 ug extracts of MCF7 cells using 1 ug POLR2H antibody (orb1260470). Western blot was performed from the immunoprecipitate using POLR2H antibody (orb1260470) at a dilution of 1:1000.