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Catalog Number | orb1538234 |
---|---|
Category | Antibodies |
Description | POLK/DNA Polymerase Kappa Antibody |
Species/Host | Goat |
Clonality | Polyclonal |
Tested applications | ELISA, IHC, IHC-P, WB |
Reactivity | Human |
Isotype | IgG |
Immunogen | This affinity-purified antibody was prepared from whole goat serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids 817-830 of Human POLK (polymerase (DNA directed) kappa). |
Concentration | 1 mg/ml |
Dilution range | ELISA (1:2000 - 1:10000), IHC, IHC-P (5 µg/ml), WB (1:500 - 1:3000) |
Conjugation | Unconjugated |
Target | POLK / DNA Polymerase Kappa |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Buffer/Preservatives | 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide. 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide |
Alternative names | POLK, DINP, DNA polymerase kappa, DINB1, DINB prot Read more... |
Note | For research use only |
Application notes | Further information: Immunohistochemistry: LS-B405 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B405 was determined to be 5 ug/ml. |
Expiration Date | 12 months from date of receipt. |
IF, IHC, IHC-P, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |