SERPINB2 Antibody

SKU: orb345658

Description

Plasminogen Activator Inhibitor 2 antibody

Images & Validation

• Tested Applications: ELISA, IHC, WB
• Dilution Range: ELISA: 1:100,000 – 150,000, IHC: 10ug/mL, WB: 1:500 – 1:2,000
• Reactivity: Human
• Application Notes:
  This affinity purified antibody has been tested for use in ELISA, Immunohistochemistry, and western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band 42-46.6 kDa in size corresponding to PAI-2 protein by western blotting in the appropriate cell lysate or extract.

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a peptide corresponding to an internal area of human PAI-2 protein.
• Target: SERPINB2
• Purity: This affinity-purified antibody is directed against human PAI-2 protein. The product was affinity purified from monospecific antiserum by immunoaffinity purification. Reactivity occurs against human PAI-2 protein. A BLAST analysis was used to suggest cross reactivity with PAI-2 proteins from several primates based on 94% homology with the immunizing sequence. Reactivity against homologues from other sources is not known.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
• Form/Appearance: Liquid (sterile filtered)
• Buffer/Preservatives: Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Concentration: 1.2 mg/mL
• Expiration Date: 12 months from date of receipt.
• Dry Ice Shipping: Please note: This product requires shipment on dry ice. A dry ice surcharge will apply.
• Disclaimer: For research use only

Alternative Names

rabbit anti-PAI-2 antibody, rabbit anti-plasminogen activator inhibitor 2 antibody, Placental plasminogen activator inhibitor, Monocyte Arg-serpin, Urokinase inhibitor, SERPINB2, Serpin B2, serpin peptidase inhibitor, PAI2, PLANH2

Immunohistochemistry of rabbit anti-Plasminogen Activator 2 antibody. Tissue: heart. Fixation: formalin fixed paraffin embedded. Antigen retrieval: not required. Primary antibody: Anti-Plasminogen Activator 2 at 10 µg/ml for 1 h at RT. Secondary antibody: Peroxidase rabbit secondary antibody at 1:10000 for 45 min at RT. Staining: Plasminogen Activator II as precipitated red signal with hematoxylin purple nuclear counterstain.

Western blot using Biorbyt's affinity purified anti-PAI-2 antibody shows detection of endogenous PAI-2 in 50 µg of HeLa whole cell lysates (p/n orb348668). The band at ~42kDa (arrowhead) corresponds to PAI-2. Faint non-specific bands are also noted at lower and higher molecular weights positions. Primary antibody was used at a 1:500 dilution in 5% BLOTTO (p/n orb348624) in PBS reacted overnight at 4°C. The membrane was washed and reacted with a 1:5000 dilution of HRP-conjugated Gt-a-Rabbit IgG (p/n orb347654) for 45 min at room temperature with ECL used for detection. Molecular weight estimation was made by comparison to prestained MW markers.

Quick Database Links

Gene Symbol

SERPINB2

UniProt

P05120

RefSeq:4505595