PIP Antibody

SKU: orb1247921

Description

PIP Antibody

Research Area

Signal Transduction

Images & Validation

• Tested Applications: ELISA, IHC, WB
• Reactivity: Human
• Application Notes:
  Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 14kDa band observed in lysates of cell line A431 (calculated MW of 16.6kDa according to NP_002643.1). Recommended concentration: 0.3-1µg/mL.Immunohistochemistry:In paraffin embedded Human Breast shows staining of luminal epithelial cells of the lactiferous duct. Recommended concentration: 2-3µg/mL.

Key Properties

• Antibody Type: Primary Antibody
• Host: Goat
• Clonality: Polyclonal
• Immunogen: The immunogen for this antibody is: C-GANKAQDNTRK
• Target: PIP
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Concentration: 500 ug/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

PIP, GCDFP15, prolactin-induced protein , HGNC:8993, GCDFP-15, GP17, GPIP4, prolactin-inducible protein, gross cystic disease fluid protein 15

orb1247921 (0.3 ug/ml) staining of A431 cell lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

orb1247921 (2.5 ug/ml) staining of paraffin embedded Human Breast. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Quick Database Links

Gene Symbol

PIP

UniProt

P12273

RefSeq (Protein):NP_002643.1