Pioglitazone hydrochloride

SKU: orb1310827

Description

Pioglitazone hydrochloride is an orally active thiazolidinedione used as an antidiabetic agent. It also exhibits potential antineoplastic properties, making it a compound of interest for research in oncology and metabolic disease studies, both in vitro and in vivo.

Research Area

Cell Biology, Metabolism Research, Molecular Biology

Key Properties

• CAS Number: 112529-15-4
• MW: 392.90
• Purity: >99.99% (May vary between batches)
• Formula: C₁₉H₂₀N₂O₃S·HCl
• SMILES: Cl.CCc1ccc(CCOc2ccc(CC3SC(=O)NC3=O)cc2)nc1
• Target: PPAR,Ferroptosis
• Solubility: 10% DMSO+40% PEG300+5% Tween 80+45% Saline:2 mg/mL (5.09 mM);DMSO:250 mg/mL (636.29 mM)

Bioactivity

• Target IC50:
  PPARγ (human):0.93 μM (EC50)|PPARδ:0.01 μM (EC50, human)|PPARγ (mouse):0.99 μM (EC50)
• In Vivo:
  Pioglitazone protects dopaminergic neurons from LPS-induced damage by inhibiting the expression of iNOS and the production of NO. It also suppresses the phosphorylation of p38 protein induced by lipopolysaccharides.
• In Vitro:
  In male obese rats, oral administration of Pioglitazone (0.3-3 mg/kg) over a period of 7 days resulted in a dose-dependent reduction of hyperglycemia, hyperlipidemia, and hyperinsulinemia.
• Cell Research:
  In order to evaluate cell proliferation, HIT-T15 cells are seeded on 96-well plates (3×104 cells/well) and cultured for 5 days as described. Viable cells are determined using the Cell Titer 96 Aqueous One Solution Cell Proliferation Assay. To evaluate cell apoptosis and cell necrosis, HIT-T15 cells are plated on 6-well dishes (7×105 cells/well) for 5 days in standard conditions (CTR) or in the presence of AGEs (AGEs) with or without Pioglitazone (0.5 or 1 μM) or AG (1 mM). They are then processed to measure both the activity of caspase-3 and the activity of lactate dehydrogenase (LDH) (a stable cytosolic enzyme that is a marker of cell membrane damage and cell death due to necrosis) using Cytotox 96 Non Radioactive Cytotoxicity Assay.

Storage & Handling

• Storage: Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

U 72107A, U-72107E, PPAR, Peroxisome proliferator-activated receptors, Pioglitazone, Pioglitazone HCl, Pioglitazone hydrochloride, Pioglitazone Hydrochloride, AD4833, AD-4833, AD 4833, Ferroptosis, human PPARγ, mouse PPARγ, Inhibitor, inhibit

Compound Identifiers

PubChem CID

60560