PI4KB Antibody

SKU: orb345806

Description

PI4K beta antibody

Research Area

Metabolism Research

Images & Validation

• Tested Applications: ELISA, IHC, WB
• Dilution Range: ELISA: 1:10,000, IHC: 5 µg/mL, WB: 1ug/ml
• Reactivity: Human
• Application Notes:
  Anti-PI4K beta antibody has been tested by ELISA, IHC, and Western Blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately ~91.4kDa corresponding to the appropriate cell lysate or extract.

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Anti-PI4K beta affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region of human PI4K beta protein.
• Target: PI4KB
• Purity: Anti-PI4K beta was affinity purified from monospecific antiserum by immunoaffinity chromatography. A BLAST analysis was used to suggest cross-reactivity with bat, rat, primate, mouse, and human based on 100% sequence homology. Cross-reactivity with PI4K beta from other sources has not been determined.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Form/Appearance: Liquid (sterile filtered)
• Buffer/Preservatives: Preservative: 40% (v/v) Glycerol; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Concentration: 1.0 mg/mL
• Expiration Date: 12 months from date of receipt.
• Dry Ice Shipping: Please note: This product requires shipment on dry ice. A dry ice surcharge will apply.
• Disclaimer: For research use only

Alternative Names

rabbit anti-PI4K beta antibody, Phosphatidylinositol 4-kinase beta, PtdIns 4-kinase beta, PI4Kß, PIK4CB, PI4KB, NPIK, PI4K92, PI4K-beta

Immunohistochemistry of Rabbit anti-PIK4CB / PI4KB antibody. Tissue: Liver. Fixation: formalin fixed paraffin embedded. Antigen retrieval: not required. Primary antibody: PIK4CB / PI4KB antibody at 5 µg/ml for 1 h at RT. Secondary antibody: Peroxidase rabbit secondary antibody at 1:10000 for 45 min at RT. Staining: PIK4CB / PI4KB as precipitated red signal with hematoxylin purple nuclear counterstain.

Quick Database Links

Gene Symbol

PI4KB

UniProt

Q9UBF8