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Phosphothreonine Antibody

SKU: orb613991

Description

Rabbit Anti-Phosphothreonine Polyclonal

Research Area

Protein Biochemistry, Signal Transduction

Images & Validation

Tested ApplicationsELISA, ICC, IF, IP, WB
Dilution RangeWB (1:500), ICC/IF (1:60), ELISA (1:2000), IP (1:100)
ReactivityAll
Application Notes
2 µg/ml was sufficient for detection of phosphorylation signal in western blot analysis using mouse spleen extract treated with Vanadium.

Key Properties

HostRabbit
ClonalityPolyclonal
ImmunogenPhosphothreonine conjugated to KLH
TargetPhosphothreonine
PurificationPeptide Affinity Purified
ConjugationUnconjugated

Storage & Handling

StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/PreservativesPBS, 50% glycerol, 0.01% sodium azide. Storage buffer changes when conjugated.
Concentration0.25 mg/ml
Expiration Date12 months from date of receipt.
DisclaimerFor research use only

Alternative Names

O-phospho-L-threonine, Phospho-threonine

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Protocol Information

WB
Western Blot (IB, immunoblot)
View Protocol
IF
Immunofluorescence
View Protocol
ICC
Immunocytochemistry
View Protocol
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
View Protocol
IP
Immunoprecipitation
View Protocol

Available Sizes

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400 μl
$ 600.00
Free Secondary Antibody (20 ul)0/0

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DispatchUsually dispatched within 2-3 weeks
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