Phosphothreonine Antibody

SKU: orb613991

Description

Rabbit Anti-Phosphothreonine Polyclonal

Research Area

Protein Biochemistry, Signal Transduction

Images & Validation

• Tested Applications: ELISA, ICC, IF, IP, WB
• Dilution Range: WB (1:500), ICC/IF (1:60), ELISA (1:2000), IP (1:100)
• Reactivity: All
• Application Notes:
  2 µg/ml was sufficient for detection of phosphorylation signal in western blot analysis using mouse spleen extract treated with Vanadium.

Key Properties

• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Phosphothreonine conjugated to KLH
• Target: Phosphothreonine
• Purification: Peptide Affinity Purified
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS, 50% glycerol, 0.01% sodium azide Storage buffer changes when conjugated
• Concentration: 0.25 mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

O-phospho-L-threonine, Phospho-threonine

Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-Phosphothreonine Polyclonal Antibody. Tissue: Cervical cancer cell line (HeLa). Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rabbit Anti-Phosphothreonine Polyclonal Antibody at 1:60 for 12 hours at 4°C. Secondary Antibody: FITC Goat Anti-Rabbit (green) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Cytoplasm. Nucleus. Magnification: 100x. (A) DAPI (blue) nuclear stain. (B) Anti-Phosphothreonine Antibody. (C) Composite.

Western blot analysis of Mouse brain cell lysates showing detection of Phosphothreonine protein using Rabbit Anti-Phosphothreonine Polyclonal Antibody. Primary Antibody: Rabbit Anti-Phosphothreonine Polyclonal Antibody at 1:1000. Left: Treated with Vanadium, Right: Non-treated.

Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-Phosphothreonine Polyclonal Antibody. Tissue: Cervical cancer cell line (HeLa). Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rabbit Anti-Phosphothreonine Polyclonal Antibody at 1:60 for 12 hours at 4°C. Secondary Antibody: R-PE Goat Anti-Rabbit (yellow) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Cytoplasm. Nucleus. Magnification: 20x. (A) DAPI (blue) nuclear stain. (B) Anti-Phosphothreonine Antibody. (C) Composite.

Quick Database Links

Gene Symbol

Phosphothreonine