Phosphothreonine Antibody

SKU: orb420306

Description

Phosphothreonine antibody

Images & Validation

• Tested Applications: ELISA, IP, WB
• Dilution Range: ELISA: 0.5 ug/ml, IP: 10.0 ug/mg sample, WB: 2.0 ug/ml
• Reactivity: Mouse
• Application Notes:
  This affinity-purified antibody is tested for use in ELISA, western blotting and immunoprecipitation assays. The antibody reacts specifically with phosphothreonine and shows minimal reactivity by ELISA and western blotting with proteins containing phosphotyrosine or phosphoserine residues. The antibody reacts with free phosphothreonine, phosphothreonine conjugated to carriers such as thyroglobulin or BSA, and detects the presence of phosphothreonine in proteins of both unstimulated and stimulated cell lysates. Although not tested, this antibody is likely functional in RIA, flow cytometry and immunohistochemistry.

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: PhosphoThreonine antibody was prepared from whole rabbit serum produced by repeated immunizations with phosphothreonine conjugated KLH.
• Purity: Antibody was prepared from monospecific antiserum by immunoaffinity chromatography using phosphorylated threonine peptide coupled to agarose. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit IgG. Reactivity is specific for phosphothreonine and minimal cross reactivity is observed against phosphotyrosine or phosphoserine.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Form/Appearance: Liquid (sterile filtered)
• Buffer/Preservatives: Preservative: None. Stabilizer: 50% (v/v) Glycerol; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Concentration: 1.0 mg/mL
• Expiration Date: 12 months from date of receipt.
• Dry Ice Shipping: Please note: This product requires shipment on dry ice. A dry ice surcharge will apply.
• Disclaimer: For research use only

Alternative Names

PhosphoThreonine (pT) reactive antibody PhosphoThreonine (pT) polyclonal antibody, PhosphoThreonine antibody produced in rabbit

Biorbyt's Affinity Purified anti-phosphothreonine (pT) antibody is shown to detect phosphorylated proteins present in mouse spleen cell lysates. Left Lane: extract of spleen from mouse treated with vanadium. Right lane: extract of spleen from untreated mouse.