Phospho-SMAD4(T277) Antibody

• Catalog Number: orb1931223
• Category: Antibodies
• Description: Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Clone Number: RB07971
• Tested applications: WB
• Predicted Reactivity: Rat
• Reactivity: Human, Mouse
• Isotype: Rabbit IgG
• Antibody Type: Primary Antibody
• Dilution range: WB: 1:500
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Conjugation: Unconjugated
• MW: 60439 Da
• Target: This SMAD4 Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding T277 of human SMAD4.
• UniProt ID: Q13485
• NCBI: NP_005350.1
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: Mothers against decapentaplegic homolog 4, MAD hom
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of lysates from NIH/3T3 cell line, untreated or treated with TGF-β (100ng/ml, 30min), using Phospho-SMAD4 (T277) Antibody (upper) or Beta-actin (lower).