You have no items in your shopping cart.
Cart summary
Item 1 of 1
Phospho-Smad2 (Ser255) Recombinant Rabbit Monoclonal Antibody
Catalog Number: orb559124
| Catalog Number | orb559124 |
|---|---|
| Category | Antibodies |
| Description | Phospho-Smad2 (Ser255) Recombinant Rabbit Monoclonal Antibody |
| Species/Host | Rabbit |
| Clonality | Recombinant |
| Clone Number | 8C12 |
| Tested applications | ICC, IF, IHC-Fr, IHC-P, WB |
| Predicted Reactivity | Mouse |
| Reactivity | Human |
| Isotype | IgG |
| Immunogen | KLH conjugated Synthesised phosphopeptide derived from human Smad2 around the phosphorylation site of Ser255 TL(p-S)PV |
| Antibody Type | Recombinant Antibody |
| Concentration | 1mg/ml |
| Dilution range | WB=1:500-1000, IHC-P=1:100-500, IHC-F=1:50-200, ICC/IF=1:50-200, IF=1:50-200 |
| Form/Appearance | Liquid |
| Conjugation | Unconjugated |
| MW | 58 kDa |
| Target | SMAD2 |
| UniProt ID | Q15796 |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Buffer/Preservatives | 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol. |
| Alternative names | Smad2(S255); Smad2(phospho S255); phospho-Smad2(Se Read more... |
| Note | For research use only |
| Expiration Date | 12 months from date of receipt. |
Blocking buffer: 5% NFDM/TBST, Primary Ab, dilution: 1:1000, Primary Ab incubation condition: 2 hours at room temperature, Secondary Ab: Goat Anti-Rabbit IgG H&L (HRP), Lysate: 1: (-) HeLa, 2: (+) HeLa + OA (100 mM, 60 min) + CA (100 mM, 60 min), Protein loading quantity: 20 µg, Exposure time: 60 s, Predicted MW: 52 kDa, Observed MW: 58 kDa.
