PHGDH Antibody

SKU: orb1264496

Description

PHGDH Antibody

Research Area

Metabolism Research, Neuroscience, Signal Transduction

Images & Validation

• Tested Applications: FC, IHC-P, WB
• Reactivity: Human, Mouse
• Predicted Reactivity: Bovine, Monkey, Porcine
• Application Notes:
  For WB starting dilution is: 1:1000For IHC-P starting dilution is: 1:50~100For FACS starting dilution is: 1:10~50

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit Ig
• Immunogen: This PHGDH antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 140-168 amino acids from the N-terminal region of human PHGDH.
• Target: PHGDH
• Molecular Weight: 57 kDa
• Purification: This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: Supplied in PBS with 0.09% (W/V) sodium azide.
• Concentration: batch dependent
• Disclaimer: For research use only

Alternative Names

D-3-phosphoglycerate dehydrogenase, 3-PGDH, PHGDH, PGDH3

Western blot analysis of PHGDH Antibody in mouse brain tissue lysates (35 ug/lane)

Western blot analysis of PHGDH using rabbit polyclonal PHGDH Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the PHGDH gene (Lane 2).

Formalin-fixed and paraffin-embedded human brain tissue reacted with PHGDH Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.

Flow cytometric analysis of hela cells using PHGDH Antibody (N-term) (bottom histogram) compared to a negative control cell (top histogram) FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Quick Database Links

Gene Symbol

PHGDH

UniProt

O43175

RefSeq:O43175