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Catalog Number | orb1260423 |
---|---|
Category | Antibodies |
Description | PHF11 Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | IF, IHC, WB |
Reactivity | Human, Mouse, Rat |
Isotype | IgG |
Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 1-292 of human PHF11 (NP_001035533.1). |
Antibody Type | Primary Antibody |
Concentration | batch dependent |
Form/Appearance | Liquid |
Conjugation | Unconjugated |
MW | Observed: 34kDa |
Target | PHF11 |
UniProt ID | Q9UIL8 |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Buffer/Preservatives | Liquid |
Alternative names | PHF11, APY, BCAP, IGEL, IGER, IGHER, NY-REN-34, NY Read more... |
Note | For research use only |
Application notes | WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200 |
Expiration Date | 12 months from date of receipt. |
Western blot analysis of extracts of SW480 cells, using PHF11 antibody (orb1260423) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
Immunohistochemistry of paraffin-embedded mouse lung using PHF11 antibody (orb1260423) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of A549 cells using PHF11 antibody (orb1260423).
ELISA, FC, ICC, IF, WB | |
Human | |
Rabbit | |
Polyclonal | |
Unconjugated |
IF, IH, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |