Phenylephrine hydrochloride

SKU: orb1310599

Description

Phenylephrine hydrochloride is a selective α1-adrenergic receptor agonist. It is widely used in research to study vasoconstriction and blood pressure regulation in both in vitro cellular assays and in vivo animal models.

Research Area

Pharmacology & Drug Discovery

Key Properties

• CAS Number: 61-76-7
• MW: 203.67
• Purity: >99.99% (May vary between batches)
• Formula: C₉H₁₄ClNO₂
• SMILES: Cl.CNCC(O)C1=CC=CC(O)=C1
• Target: Adrenergic Receptor,Endogenous Metabolite
• Solubility: 10% DMSO+40% PEG300+5% Tween 80+45% Saline:2 mg/mL (9.82 mM);DMSO:252.5 mg/mL (1239.75 mM);H2O:20.4 mg/mL (100.16 mM)

Bioactivity

• In Vivo:
  Phenylephrine protects cardiomyocytes from serum deprivation and hypoxia. It induces rapid translocation of PKC-epsilon (EC 50= 0.9 mM), with a lesser fraction lost compared to ET-1, and prevents downregulation of Bcl-X and Bcl-2 mRNA/proteins while inducing hypertrophic growth. Phenylephrine dose-dependently increases contractile force in hyperosmotic cells at pCa 7, which is reversible upon phentolamine addition. The protective effects mediated by Phenylephrine are inhibited by the PI 3-kinase inhibitor wortmannin and mimicked by the caspase-9 peptide inhibitor LEHD-fmk. It stimulates phosphoinositide hydrolysis, cellular growth, and expression of various genes associated with cardiac hypertrophy, such as atrial natriuretic factor. Phenylephrine-induced NOi release requires stimulation by IP3 and PI-3K/Akt-dependent calcium signaling, and it also enhances localized sarcoplasmic reticulum calcium release through IP3-dependent signaling. Induction of NOi release by Phenylephrine is inhibited by 1 mM prazocin, 10 mM W-7, 10 mM L-NIO, 10 mM LY294002, 2 mM H-89, 5 mM thapsigargin, 10 mM ryanodine, 2 mM 2-APB, or 10 mM xestospongin C. Additionally, 1 μM Phenylephrine significantly enhances HGF-induced hepatocyte DNA synthesis and proliferation. Finally, 1 mM Phenylephrine reversibly increases peak I(Ca,L) by 51.3% (N=40) and shifts the activation voltage by -10 mV.

Storage & Handling

• Storage: keep away from direct sunlight,store under nitrogen,store at low temperature | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

(R)-(-)-Phenylephrine hydrochloride, Adrenergic Receptor, AdrenergicReceptor, EndogenousMetabolite, Endogenous Metabolite, NCI-c55641, NCI-c55641 HCL, Phenylephrine HCl, α1-adrenergic receptor

Compound Identifiers

PubChem CID

6102