Phenylephrine hydrochloride

SKU: orb1310599

Description

Phenylephrine hydrochloride is a selective α1-adrenergic receptor agonist. It is widely used in research to study vasoconstriction and blood pressure regulation in both in vitro cellular assays and in vivo animal models.

Research Area

Metabolism Research, Neuroscience, Pharmacology & Drug Discovery

Key Properties

• CAS Number: 61-76-7
• MW: 203.67
• Purity: 99.08% (May vary between batches)
• Formula: C₉H₁₄ClNO₂
• SMILES: Oc1cccc([C@H](CNC)O)c1.Cl
• Target: Endogenous Metabolite,Adrenergic Receptor
• Solubility: 10% DMSO+40% PEG300+5% Tween 80+45% Saline:2 mg/mL (9.82 mM);DMSO:252.5 mg/mL (1239.75 mM);H2O:20.4 mg/mL (100.16 mM)

Bioactivity

• In Vivo:
  Phenylephrine protects cardiomyocytes from serum deprivation and hypoxia. It induces rapid translocation of PKC-epsilon (EC 50= 0.9 mM), with a lesser fraction lost compared to ET-1, and prevents downregulation of Bcl-X and Bcl-2 mRNA/proteins while inducing hypertrophic growth. Phenylephrine dose-dependently increases contractile force in hyperosmotic cells at pCa 7, which is reversible upon phentolamine addition. The protective effects mediated by Phenylephrine are inhibited by the PI 3-kinase inhibitor wortmannin and mimicked by the caspase-9 peptide inhibitor LEHD-fmk. It stimulates phosphoinositide hydrolysis, cellular growth, and expression of various genes associated with cardiac hypertrophy, such as atrial natriuretic factor. Phenylephrine-induced NOi release requires stimulation by IP3 and PI-3K/Akt-dependent calcium signaling, and it also enhances localized sarcoplasmic reticulum calcium release through IP3-dependent signaling. Induction of NOi release by Phenylephrine is inhibited by 1 mM prazocin, 10 mM W-7, 10 mM L-NIO, 10 mM LY294002, 2 mM H-89, 5 mM thapsigargin, 10 mM ryanodine, 2 mM 2-APB, or 10 mM xestospongin C. Additionally, 1 μM Phenylephrine significantly enhances HGF-induced hepatocyte DNA synthesis and proliferation. Finally, 1 mM Phenylephrine reversibly increases peak I(Ca,L) by 51.3% (N=40) and shifts the activation voltage by -10 mV.
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Storage & Handling

• Storage: -20°C
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

(R)-(-)-Phenylephrine hydrochloride, Adrenergic Receptor, AdrenergicReceptor, EndogenousMetabolite, Endogenous Metabolite, NCI-c55641, NCI-c55641 HCL, Phenylephrine HCl, α1-adrenergic receptor

Compound Identifiers

PubChem CID

6102