Pepsin Antibody

SKU: orb344342

Description

Pepsin antibody

Images & Validation

• Tested Applications: ELISA, IHC, IP, WB
• Dilution Range: ELISA: 1:50:000 - 1:200,000, IHC: 1:2,000 - 1:10,000, IP: 1:100, WB: 1:5,000 - 1:20,000
• Reactivity: Porcine
• Application Notes:
  Anti-Pepsin antibody has been tested in ELISA, western blot and immunohistochemistry. This product detects a single band of the expected apparent molecular weight and for ELISA. Researchers should determine optimal titers for applications that are not stated below.

Key Properties

• Antibody Type: Primary Antibody
• Host: Goat
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Anti-Pepsin Antibody was produced by repeated immunizations with pork Pepsin protein isolated from swine stomach.
• Purity: Anti-PEPSIN is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum as well as purified and partially purified Pepsin [Porcine Stomach]. Cross reactivity against Pepsin from other tissues and species may occur but have not been specifically determined.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Form/Appearance: Lyophilized
• Buffer/Preservatives: Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Concentration: 1.0 mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

goat anti-Pepsin Antibody, Pepsin A antibody, Pepsin a precursor antibody, Pepsin antibody, Pepsinogen 1 second locus antibody, Pepsinogen 3 group 1 antibody, Pepsinogen a3 antibody

Immunohistochemistry of goat anti-pepsin antibody at 1:100.

Western Blot of Anti-Pepsin Antibody. Lane 1: Opal Prestained Molecular Weight Ladder. Lane 2: HeLa Lysate (10 µg) + Pepsin (0.1 µg) [+]. Lane 3: HeLa Lysate (10 µg) + Pepsin (0.05 µg) [+]. Lane 4: HeLa Lysate (10 µg) + Pepsin (0.02 µg) [+]. Lane 5: HeLa Lysate (p/n orb348668) (10 µg) [-]. Primary Antibody: Anti-Pepsin at 1 µg/ml overnight at 2-8°C. Secondary Antibody: Donkey Anti-Goat IgG HRP at 1:40000 at RT for 30 minutes. Block: BlockOut Buffer (p/n orb348644). Exposure: 15 sec. Predicted MW: ~41kDa. Observed MW: ~45kDa.

Quick Database Links

UniProt

P00791

RefSeq:AAA31095.1