PDK2 Antibody

SKU: orb1939394

Description

Mouse Monoclonal Antibody (Mab)

Research Area

Signal Transduction

Images & Validation

• Tested Applications: IHC-P, WB
• Dilution Range: WB - 1:1000, IHC-P - 1:25
• Reactivity: Human, Mouse

Key Properties

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1,K
• Clone No.: B302EV32X4X5
• Immunogen: This PDK2 monoclonal antibody is generated from mouse immunized with PDK2 recombinant protein.
• Target: PDK2
• Molecular Weight: 46154 Da
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Form/Appearance: Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

[Pyruvate dehydrogenase (acetyl-transferring)] kinase isozyme 2, mitochondrial, Pyruvate dehydrogenase kinase isoform 2, PDH kinase 2, PDKII, PDK2, PDHK2

All lanes: Anti- at 1:1000 dilution. Lane 1: human heart lysate. Lane 2: human skeletal muscle lysate.Lysates/proteins at 20 µg per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 46 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Anti-PDK2 Antibody at 1:1000 dilution + human heart lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 46 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Staining PDK2 in human liver sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0.5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary Antibody.

PDK2 Antibody western blot analysis in mouse cerebellum tissue lysates (35 μg/lane). This demonstrates the PDK2 antibody detected the PDK2 protein (arrow).

Western blot analysis of lysates from human skeletal muscle, human brain, mouse brain, rat brain tissue (from left to right), using PDK2 Antibody. diluted at 1:2000 at each lane. A goat anti-mouse IgG H&L (HRP) at 1:3000 dilution was used as the secondary Antibody. Lysates at 20 μg per lane.

Quick Database Links

Gene Symbol

PDK2

UniProt

Q15119

RefSeq (Protein):NP_002602.2, NP_001186827.1, NP_001186828.1