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Catalog Number | orb1256710 |
---|---|
Category | Antibodies |
Description | PDHB Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | WB |
Reactivity | Human, Mouse, Rat |
Isotype | IgG |
Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human PDHB (NP_000916.2). |
Concentration | batch dependent |
Dilution range | WB: 1:500 - 1:2000 |
Form/Appearance | Liquid |
Conjugation | Unconjugated |
MW | Observed: 39kDa |
Target | PDHB |
UniProt ID | P11177 |
Storage | Store at -20˚°C. Avoid freeze / thaw cycles. |
Buffer/Preservatives | Liquid |
Alternative names | PDHB, DKFZp564K0164, PHE1B, PDHBD, PDHE1-B Read more... |
Note | For research use only |
Application notes | WB: 1:500 - 1:2000 |
Expiration Date | 12 months from date of receipt. |
Western blot analysis of extracts of various cell lines, using PDHB antibody (orb1256710) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
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