PDE5A Antibody

• Catalog Number: orb1247128
• Category: Antibodies
• Description: PDE5A Antibody
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, FC, IF, WB
• Predicted Reactivity: Bovine, Canine, Rat
• Reactivity: Human
• Immunogen: The immunogen for this antibody is: C-LINGESGQAKRN
• Concentration: 500 ug/mL
• Dilution range: Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:In transfected HEK293 transiently expressing PDE5A isoform 2 a band of approx. 95kDa is observed. This band is not observed in the non-transfected HEK293 (calculated MW of 94.8kDa according to isoform 2, NP_236914.2). Recommended concentration: 0.3-1ug/ml.Immunofluorescence: Strong expression of the protein seen in the cytoplasm of HeLa cells. Recommended concentration: 10ug/ml. Flow Cytometry: Flow cytometric analysis of HEK293 cells. Recommended concentration: 10ug/ml.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: PDE5A
• UniProt ID: O76074
• NCBI: NP_246273.2, NP_236914.2, NP_237223, NP_001074.2
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: PDE5A, CN5A, PDE5, PDE5A1, CGB-PDE, phosphodiester
• Note: For research use only
• Application notes: Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:In transfected HEK293 transiently expressing PDE5A isoform 2 a band of approx. 95kDa is observed. This band is not observed in the non-transfected HEK293 (calculated MW of 94.8kDa according to isoform 2, NP_236914.2). Recommended concentration: 0.3-1ug/ml.Immunofluorescence: Strong expression of the protein seen in the cytoplasm of HeLa cells. Recommended concentration: 10ug/ml. Flow Cytometry: Flow cytometric analysis of HEK293 cells. Recommended concentration: 10ug/ml.
• Expiration Date: 12 months from date of receipt.

HEK293 overexpressing PDE5A and probed with orb1247128 (mock transfection in first lane).

orb1247128 Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (2 ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI.

orb1247128 Flow cytometric analysis of paraformaldehyde fixed HEK293 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (1 ug/ml). IgG control: Unimmunized goat IgG (black line).