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PAX3 Rabbit Polyclonal Antibody
Description
Research Area
Images & Validation
−| Tested Applications | IHC, WB |
|---|---|
| Reactivity | Human, Mouse |
| Predicted Reactivity | Bovine, Canine, Equine, Guinea pig, Rabbit, Rat, Zebrafish |
Key Properties
−| Host | Rabbit |
|---|---|
| Clonality | Polyclonal |
| Immunogen | The immunogen is a synthetic peptide directed towards the C terminal region of human PAX3 |
| Target | PAX3 |
| Protein Sequence | Synthetic peptide located within the following region: GGVPHQPQTDYALSPLTGGLEPTTTVSASCSQRLDHMKSLDSLPTSQSYC |
| Molecular Weight | 53kDa |
| Purification | Affinity Purified |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Buffer/Preservatives | Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose. |
| Concentration | 0.5 mg/ml |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
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Sample Type: Mouse B16F10, Primary Antibody dilution: 1:200, Secondary Antibody: Goat anti-rabbit-FITC, Secondary Antibody dilution: 1:800, Color/Signal Descriptions: Green: FITC Blue: DAPI, Gene Name: PAX3.

WB Suggested Anti-PAX3 Antibody, Positive Control: Lane 1: Flag-PAX3(overexpression, human), HEK293, 50?g, Lane 2: Mouse, B16F10, 50?g, Lane 3: Human, A375, 50?g. Lane 4: Human, A2058, 50?g, Primary Antibody Dilution: 1:5000, Secondary Antibody: Goat anti-rabbit AP, Secondry Antibody Dilution: 1:5000.

WB Suggested Anti-PAX3 Antibody Titration: 0.2-1 ug/ml, ELISA Titer: 1:1562500, Positive Control: Human Lung.
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Protocol Information
PAX3 Rabbit Polyclonal Antibody (orb574360)
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