PAX3 Rabbit Polyclonal Antibody

• Catalog Number: orb574208
• Category: Antibodies
• Description: Rabbit polyclonal antibody to PAX3
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: WB
• Predicted Reactivity: Bovine, Canine, Equine, Guinea pig, Rabbit, Rat, Yeast
• Reactivity: Human, Mouse
• Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human PAX3
• Concentration: 0.5 mg/ml
• Form/Appearance: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
• Conjugation: Unconjugated
• MW: 53kDa
• Target: PAX3
• UniProt ID: Q494Z3
• Protein Sequence: Synthetic peptide located within the following region: DRNTVPSVSSISRILRSKFGKGEEEEADLERKEAEESEKKAKHSIDGILS
• NCBI: NP_852123
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
• Alternative names: WS1, WS3, CDHS, HUP2
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Sample Tissue: Mouse Small Intestine, Antibody dilution: 1 ug/ml.

Sample Tissue: Human Jurkat Whole Cell, Antibody dilution: 1 ug/ml.

Lanes: Lane 1: 20 ug RH30 lysate, Lane 2: 20 ug RH30 lysate, Lane 3: 20 ug RH41 lysate, Lane 4: 20 ug RH41 lysate, Primary Antibody dilution: 1:1000, Secondary Antibody: Donkey anti-rabbit HRP, Secondary Antibody dilution: 1:4000, Gene Name: PAX3.

WB Suggested Anti-PAX3 Antibody Titration: 0.2-1 ug/ml, Positive Control: Hela cell lysate.