Pak1 Antibody

SKU: orb1273558

Description

Pak1 Antibody

Images & Validation

• Tested Applications: WB
• Reactivity: Bovine, Canine, Human, Mouse, Rat
• Application Notes:
  The antibody has been directly tested for reactivity in Western blots with rat tissue. It is anticipated that the antibody will react with bovine, canine, human and mouse based on the fact that these species have 100% homology with the amino acid sequence used as antigen.

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Phosphopeptide corresponding to amino acid residues surrounding the phospho-Thr402 of rat p21 activated kinase 2 (PAK-2). The peptide sequence used is identical in PAK-1, 2 and 3. Note: Thr402 in PAK-2 corresponds to Thr423 in human PAK-1.
• Target: Pak1
• Molecular Weight: 68/70
• Purification: Affinity Purified
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Concentration: batch dependent
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Western blot of rat hippocampal lysate showing specific immunolabeling of the ~68k to ~70k PAK protein (Control). The phosphospecificity of this labeling is shown in the second lane (lambda-phosphatase: lamda-Ptase). The blot is identical to the control except that it was incubated in lamda-Ptase (1200 units for 30 min) before being exposed to the phospho-Thr402 PAK-1, 2, 3 antibody. The immunolabeling of PAK is completely eliminated by treatment with lamda-Ptase.

Quick Database Links

Gene Symbol

Pak1

UniProt

P35465

RefSeq:P35465