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Description
Research Area
Metabolism Research
Images & Validation
−
Key Properties
−| Source | HEK293 cells |
|---|---|
| Biological Activity | Biological activity was measured by its ability to cleave a PAF analog in a chromogenic substrate linked assay. At a PAF - AH concentration of 10.0 μg/mL, 50% cleavage was achieved at an incubation time of approximately 2 minutes. |
| Target | PLA2G7 |
| Purity | Greater than 95% by SDS-PAGE gel and HPLC analyses.Endotoxin level is less than 0.1 ng per μg (1EU/μg). |
| Protein Sequence | FDWQYINPVA HMKSSAWVNK IQVLMAAASF GQTKIPRGNG PYSVGCTDLM FDHTNKGTFL RLYYPSQDND RLDTLWIPNK EYFWGLSKFL GTHWLMGNIL RLLFGSMTTP ANWNSPLRPG EKYPLVVFSH GLGAFRTLYS AIGIDLASHG FIVAAVEHRD RSASATYYFK DQSAAEIGDK SWLYLRTLKQ EEETHIRNEQ VRQRAKECSQ ALSLILDIDH GKPVKNALDL KFDMEQLKDS IDREKIAVIG HSFGGATVIQ TLSEDQRFRC GIALDAWMFP LGDEVYSRIP QPLFFINSEY FQYPANIIKM KKCYSPDKER KMITIRGSVH QNFADFTFAT GKIIGHMLKL KGDIDSNVAI DLSNKASLAF LQKHLGLHKD FDQWDCLIEG DDENLIPGTN INTTNQHIML QNSSGIEKYN |
Storage & Handling
−| Storage | The lyophilized PAF-AH recombinant protein is stable for at least 2 years from date of receipt at -20°C. Reconstituted PAF-AH is stable for at least 3 months when stored in working aliquots with a carrier protein at -20°C. As with any protein, exposing PAF-AH recombinant protein to repeated freeze / thaw cycles is not recommended. When working with proteins care should be taken to keep recombinant protein at a cool and stable temperature. |
|---|---|
| Form/Appearance | Lyophilized |
| Expiration Date | 6 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−PAFAD, PAFAH, LP-PLA2, LDL-PLA2, Platelet-activating factor acetylhydrolase, 1-alkyl-2-acetylglycerophosphocholine esterase, PAF acetylhydrolase
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Quick Database Links
UniProt Details
− No UniProt data available
NCBI Reference Sequences
−Associated Accession Numbers
Curated reference sequences for the gene transcript and protein product| Protein | NP_001161829.1 |
|---|
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Protein Handling and Storage Guide
Protein Handling Guide
PAF-AH Recombinant Protein (orb1230961)
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