bmpA, BB_0383 Antibody

SKU: orb344680

Description

p39 antibody

Images & Validation

• Tested Applications: ELISA, WB
• Dilution Range: ELISA: >1:5,000, WB: 1:1,000
• Reactivity: Bacteria
• Application Notes:
  This protein-A purified antibody has been tested for use in ELISA and Western blotting. Specific conditions for reactivity should be optimized by the user. Expect a band approximately 35.4 kDa in size corresponding to Borrelia burgdorferi p39 protein by Western blotting in the appropriate cell lysate or extract.

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: MBP-fusion protein corresponding to Borrelia burgdorferi p39 protein.
• Target: bmpA, BB_0383
• Purity: This product was Protein-A purified and cross-adsorbed against MBP from monospecific antiserum by chromatography. This antibody is specific for Borrelia burgdorferi p39 protein. A BLAST analysis was used to suggest cross-reactivity with p39 from B. burgdorferi and B. afzelii sources based on 100% homology with the immunizing sequence. Partial reactivity is expected against B. garinii sources based on 88-95% homology and to B.spielmanii A14S based on 86-96% homology. Cross-reactivity with p39 from other sources has not been determined.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Form/Appearance: Lyophilized
• Buffer/Preservatives: Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Concentration: 1.0 mg/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

rabbit anti-p39 Antibody, Basic membrane protein A, Borrelia burgdorferi bmpA, immunodominant antigen P39, membrane lipoprotein BmpA

Western blot showing detection of 0.1 µg of recombinant p39 protein. Lane 1: Molecular weight markers. Lane 2: MBP-p39 fusion protein (expected MW: 77.8 kDa). Lane 3: MBP alone. Protein was run on a 4-20% gel, then transferred to 0.45 µm nitrocellulose. After blocking with 1% BSA-TTBS (p/n orb348540, diluted to 1X) overnight at 4°C, primary antibody was used at 1:1000 at room temperature for 30 min. HRP-conjugated Goat-Anti-Rabbit (p/n orb347654) secondary antibody was used at 1:40000 in orb348637 blocking buffer.

Quick Database Links

Gene Symbol

bmpA, BB_0383

UniProt

Q45010

RefSeq (Protein):WP_002656850.1