OAC1

SKU: orb1225394

Description

OAC1 is a Octamer-binding transcription factor 4 (Oct4)-activating compound; enhances the iPSC reprogramming efficiency and accelerated the reprogramming process.

Key Properties

• CAS Number: 300586-90-7
• MW: 237.26
• Purity: >98% (HPLC)
• Formula: C₁₄H₁₁N₃O
• SMILES: O=C(NC1=CC2=C(NC=C2)C=N1)C3=CC=CC=C3
• Target: OCT
• Solubility: Ethanol: 21 mg/mL (88.51 mM); DMSO: 47 mg/mL (198.09 mM)

Bioactivity

• In Vivo:
  OAC1 (50000 CB CD34+ cells with OAC1(500 nM); i.h.; 16 weeks) enhances short and long-term engrafting hematopoietic stem cells (HSC) in irradiated NSG mice. Animal model: Irradiated NSG mice with OAC1 or vehicle control treated CB CD34+ cells within 24h after irradiation. Dosage: 16 weeks. Administration: Subcutaneous injection OAC1(500 nM) treated CB CD34+ cells. Result: Increased cord blood (CB) cells in primary recipients, compared to the vehicle control group, with the board increase for human B cells, T cells, and myeloid cells in the bone marrow (BM) of primary recipients, resulting in a significant expansion of SRC numbers.
• In Vitro:
  OAC1 (10 μM; 7 d) enhances reprogramming efficiency. OAC1 increases induced pluripotent stem cells (iPSC) generation from mouse embryonic fibroblasts (MEFs) and accelerates the appearance of iPSC-like colonies. OAC1 (1 μM; 2 d; human IMR90 fibroblast cells) activates endogenous Oct4, Nanog, Sox2, and Tet1 expression. OAC1 (500 nM; 4 d; CD34+ cells) increases numbers of phenotypic hematopoietic stem cells (HSC) and functional Hematopoietic progenitor cells (HPC). OAC1 (500 nM; 4 d; CD34+ cells) activates OCT4 through upregulation of HOXB4 expression to expand hematopoietic stem cells (HSC). Cell Proliferation Assay Cell line: CD34+ cells from human cord blood. Concentration: 500 nM. Incubation time: 4 days. Result: Increased the numbers of CD34+CD38- cells. Increased the number of Lin-CD34+CD38-CD45RA-CD90+CD49f+ cells. Increased in the number of phenotypic hematopoietic stem cells (HSC) compared to control vectors. Enhanced expansion of HPC with both full and suboptimal cytokine doses in the expansion and colony forming phases.

Storage & Handling

• Storage: Storage temperature: -20°C. Stability: ≥ 2 years
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

OAC 1 | OAC1 | OAC-1

Quick Database Links

Gene Symbol

OCT