NUDT21 Recombinant Mouse Monoclonal Antibody

SKU: orb1173159

Description

NUDT21 Recombinant Mouse Monoclonal Antibody

Images & Validation

• Tested Applications: ICC, IF, IHC-Fr, IHC-P, WB
• Dilution range: WB=1:500-1000, IHC-P=1:100-500, IHC-F=1:100-500, ICC/IF=1:50-200, IF=1:100-500
• Reactivity: Human, Mouse
• Predicted Reactivity: Human, Mouse

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Recombinant
• Isotype: IgG1, Kappa
• Clone No.: H8B8
• Target: NUDT21
• Molecular Weight: 27 kDa
• Purification: Affinity purified by Protein G
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Disclaimer: For research use only

Alternative Names

CFIM 25; CFIM25; Cleavage and polyadenylation specific factor 5 25 kD subunit; Cleavage and polyadenylation specific factor 5 25 kDa; Cleavage and polyadenylation specific factor 5; Cleavage and polyadenylation specificity factor 25 kDa subunit; Cleavage and polyadenylation specificity factor subunit 5; Cleavage factor Im complex 25 kDa subunit; CPSF 25; CPSF 25 kDa subunit; CPSF 5; CPSF25; CPSF5; CPSF5_HUMAN; DKFZp686H1588; Nucleoside diphosphate linked moiety X motif 21; Nucleoside diphosphate-linked moiety X motif 21; Nudix(nucleoside diphosphate linked moiety X) type motif 21; Nudix motif 21; NUDT 21; Pre mRNA cleavage factor Im(25kD); Pre mRNA cleavage factor Im 25 kDa subunit; Pre mRNA cleavage factor Im 25kD subunit; Pre-mRNA cleavage factor Im 25 kDa subunit.

Blocking buffer: 5% NFDM/TBST, Primary ab Dilution: 1:1000, Primary ab incubation condition: room temperature 2h, Secondary ab: Goat Anti-Mouse IgG H&L (HRP), Lysate: HeLa, 3T3, A431, N2a, Protein loading quantity: 20 µg, Exposure time: 30 s, Predicted MW: 27 kDa, Observed MW: 27 kDa.

Cell line: HepG2, Fixative: 100% Ice-cold methanol, Permeabilization: 0.1% TritonX-100, Primary ab Dilution: 1:50, Primary incubation condition: 4°C overnight, Secondary ab: Goat Anti-Mouse IgG, Nuclear counter stain: DAPI (Blue), Counter stain: Tubulin (Red).

Tissue: Human brain, Section type: Formalin fixed & Paraffin embedded section, Retrieval method: High temperature and high pressure, Retrieval buffer: Tris/EDTA buffer, pH9.0 Primary ab Dilution: 1:100, Primary ab incubation condition: 1 hour at room temperature, Secondary ab: SP Kit (Mouse), Counter stain: Hematoxylin (Blue), Comment: Color brown is the positive signal for orb1173159.

Tissue: Mouse brain, Section type: Formalin fixed & Paraffin embedded section, Retrieval method: High temperature and high pressure, Retrieval buffer: Tris/EDTA buffer, pH9.0 Primary ab Dilution: 1:100, Primary ab incubation condition: 1 hour at room temperature, Secondary ab:SP Kit (Mouse), Counter stain: Hematoxylin (Blue), Comment: Color brown is the positive signal for orb1173159.

Quick Database Links

Gene Symbol

NUDT21