Nucleolin antibody

SKU: orb2276320

Description

Nucleolin antibody validated for immunohistochemistry on 76 different Normal Tissues

Images & Validation

• Tested Applications: IHC
• Dilution Range: 1:100-1:200
• Reactivity: Human
• Application Notes:
  Positive Control: Colon: A nuclear nucleolin immunostaining should be seen in all cells. A focus of the staining on nucleoli should be visible – at least – in the superficial epithelial cell layer. Negative Control: Colon: A cytoplasmic or membranous nucleolin immunostaining should be absent in all cells. Cellular Localization: Nuclear Protocol Recommendations: Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121°C in pH 7.8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37°C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer’s directions.

Key Properties

• Host: Rabbit
• Clonality: Monoclonal
• Isotype: IgG
• Clone No.: MSVA-623R
• Immunogen: Recombinant full-length human NCL protein
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

NCL; Nucl; Nucleolin; Protein C23

Distinct nucleolar immunostaining in the nucleoli of an esophageal squamous cell carcinoma.

Fallopian tube mucosa The entire nuclei of epithelial cells are nucleolin positive but the immunostaining is most distinct in the nucleolus.

Uterus endometrium In this sample of endometrium nucleolin staining is particularly prominent in the nucleoli of epithelial and stromal cells.

Quick Database Links

UniProt

P19338