Nucleoli Marker Antibody [NM95]

• Catalog Number: orb1252651
• Category: Antibodies
• Description: Nucleoli Marker Antibody [NM95]
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: NM95
• Tested applications: FC, ICC, IF, IHC
• Reactivity: Human
• Isotype: IgG1, kappa
• Immunogen: Nuclei of myeloid leukemia biopsy cells were used as the immunogen.
• Concentration: 0.2 mg/mL
• Dilution range: Flow Cytometry: 0.5-1ug/10^6 cellsIF: 0.5-1 ug/mlImmunocytochemistry (Acetone-fixed): 0.5-1 ug/ml for 30 min at RTIHC (Formalin-paraffin): 0.5-1 ug/ml for 30 min at RT (1)Prediluted format : incubate for 30 min at RT (2)The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the Nucleoli marker antibody to be titered up or down for optimal performance.1. Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Storage: Aliquot and Store at 2-8°C. Avoid freez-thaw cycles.
• Buffer/Preservatives: PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
• Note: For research use only
• Application notes: Flow Cytometry: 0.5-1ug/10^6 cellsIF: 0.5-1 ug/mlImmunocytochemistry (Acetone-fixed): 0.5-1 ug/ml for 30 min at RTIHC (Formalin-paraffin): 0.5-1 ug/ml for 30 min at RT (1)Prediluted format : incubate for 30 min at RT (2)The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the Nucleoli marker antibody to be titered up or down for optimal performance.1. Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
• Expiration Date: 12 months from date of receipt.

IHC staining of FFPE human skin with Nucleoli marker antibody.

IHC staining of FFPE human tonsil tissue with Nucleoli marker antibody.

Immunofluorescent testing of colon carcinoma with Alexa Fluor 488 conjugated Nucleoli marker antibody.

Intracellular FACS testing of 293 cells with Nucleoli marker antibody (green).

IHC staining of FFPE human colon carcinoma with Nucleoli Marker antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.