NSUN2 Rabbit Polyclonal Antibody

• Catalog Number: orb580531
• Category: Antibodies
• Description: Rabbit polyclonal antibody to NSUN2
• Target: NSUN2
• Clonality: Polyclonal
• Species/Host: Rabbit
• Conjugation: Unconjugated
• Reactivity: Human
• Predicted Reactivity: Bovine, Canine, Equine, Guinea pig, Mouse, Porcine, Rabbit, Rat
• Form/Appearance: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
• Concentration: 0.5 mg/ml
• Buffer/Preservatives: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
• Immunogen: The immunogen is a synthetic peptide directed towards the C terminal region of human NSUN2
• Protein Sequence: Synthetic peptide located within the following region: FINSRIITVSMEDVKILLTQENPFFRKLSSETYSQAKDLAKGSIVLKYEP
• UniProt ID: Q08J23
• MW: 86 kDa
• Tested applications: WB
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: MISU, MRT5, SAKI, TRM4
• Note: For research use only
• NCBI: NP_060225

25 ug of the indicated Human whole cell extracts was loaded onto a 12% SDS-PAGE gel. 3 ug/ml of the antibody was used in this experiment. An 85 kDa isoform also contains the peptide sequence. Protein may be modified by phosphorylation.

Positive control (+): HeLa Cell Lysate (HL), Negative control (-): Human Placenta (PL), Antibody concentration: 1 ug/ml.

WB Suggested Anti-NSUN2 Antibody Titration: 0.2-1 ug/ml, Positive Control: Hela cell lysate.