NQO1 Rabbit Polyclonal Antibody

SKU: orb499676

Description

NQO1 Rabbit Polyclonal Antibody

Research Area

Metabolism Research

Images & Validation

• Tested Applications: IF, IHC-Fr, IHC-P, WB
• Dilution Range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
• Reactivity: Human, Mouse, Rat
• Predicted Reactivity: Human, Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from mouse NQO1 (201-274/274aa)
• Target: NQO1
• Molecular Weight: 31 kDa
• Purification: Affinity purified by Protein A
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

DHQU; DIA4; DTD; NMOR1; NMORI; QR1; Nmo-1; Nmo1; Ox-1; Ox1; NADPH-d; NQO1_HUMAN; NQO1; Azoreductase; DT-diaphorase (DTD); Menadione reductase; NAD(P)H:quinone oxidoreductase 1; Phylloquinone reductase; Quinone reductase 1 (QR1); 1.6.5.2; NQO1_MOUSE; NQO1_RAT;

Paraformaldehyde-fixed, paraffin embedded (rat stomach), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (NQO1) Polyclonal Antibody, Unconjugated (orb499676) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Sample: A549 (Human) Cell Lysate at 30 ug, Primary: Anti-NQO1 (orb499676) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 31 kD, Observed band size: 31 kD.

Sample: Siha (Human) Cell Lysate at 30 ug, Primary: Anti-NQO1 (orb499676) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 31 kD, Observed band size: 31 kD.

Sample: Stomach (Mouse) Lysate at 40 ug, Primary: Anti-NQO1 (orb499676) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 31 kD, Observed band size: 31 kD.

Quick Database Links

Gene Symbol

NQO1

UniProt

Q64669