NCS1 Antibody

SKU: orb345769

Description

Neuronal Calcium Sensor 1 antibody

Research Area

Neuroscience

Images & Validation

• Tested Applications: ELISA, IHC, WB
• Dilution Range: ELISA: 1: 10,000, IHC: 5 µg/mL, WB: 1ug/ml
• Reactivity: Human
• Application Notes:
  Anti-Neuronal Calcium Sensor 1 antibody is tested for ELISA and immunohistochemistry and useful for Western Blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately ~21kDa corresponding to the appropriate cell lysate or extract.

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Neuronal Calcium Sensor 1 affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to the C-terminus of human Neuronal Calcium Sensor 1 .
• Target: NCS1
• Purity: Anti-Neuronal Calcium Sensor 1 was affinity purified from monospecific antiserum by immunoaffinity chromatography. A BLAST analysis was used to suggest cross-reactivity with human, chicken, frog, and rat based on 100% sequence homology. Cross-reactivity with Neuronal Calcium Sensor 1 from other sources has not been determined.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Form/Appearance: Liquid (sterile filtered)
• Buffer/Preservatives: Preservative: 50% (v/v) Glycerol; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Concentration: 0.42 mg/mL
• Expiration Date: 12 months from date of receipt.
• Dry Ice Shipping: Please note: This product requires shipment on dry ice. A dry ice surcharge will apply.
• Disclaimer: For research use only

Alternative Names

rabbit anti-NCS1 antibody, anti-Frequenin antibody, Neuronal calcium sensor 1, NCS-1, NCS 1, Frequenin homolog, Frequenin-like ubiquitous protein, Frequenin-like protein, FLUP, FREQ

Immunohistochemistry of Rabbit anti-NCS1 / NCS-1 antibody. Tissue: Brain, cortex. Fixation: formalin fixed paraffin embedded. Antigen retrieval: not required. Primary antibody: NCS1 / NCS-1 antibody at 5 µg/ml for 1 h at RT. Secondary antibody: Peroxidase rabbit secondary antibody at 1:10000 for 45 min at RT. Staining: NCS1 / NCS-1 as precipitated red signal with hematoxylin purple nuclear counterstain.

Quick Database Links

Gene Symbol

NCS1

UniProt

P62166