NEFL Antibody

SKU: orb1252039

Description

NEFL Antibody

Research Area

Neuroscience, Signal Transduction

Images & Validation

• Tested Applications: IF, IHC-P
• Reactivity: Human, Rat
• Application Notes:
  Immunofluorescence: 1-2 µg/mLImmunohistochemistry (FFPE): 1-2 µg/mL for 30 min at RT (1)Prediluted format: incubate for 30 min at RT (2)Optimal dilution of the Neurofilament antibody should be determined by the researcher.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1
• Clone No.: MSI27-1
• Immunogen: Recombinant human neurofilament light chain protein was used as the immunogen for the Neurofilament antibody.
• Target: NEFL
• Purification: Protein G affinity chromatography
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
• Concentration: 0.2 mg/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

NEFL, 68 kDa neurofilament protein, CMT1F, CMT2E, NF68, NF-L, NFL, Neurofilament subunit NF-L

IHC testing of FFPE human cerebellum with Neurofilament antibody (clone MSI27-1).

Quick Database Links

Gene Symbol

NEFL

UniProt

P07196