NeuroD1 Antibody (N-term)

SKU: orb1931825

Description

Purified Rabbit Polyclonal Antibody (Pab)

Research Area

Cardiovascular Research, Metabolism Research, Neuroscience

Images & Validation

• Tested Applications: IF, IHC-P, WB
• Dilution range: IF: 1:10~50, IF: 1:100, WB: 1:1000, IHC-P: 1:50~100
• Reactivity: Human

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Clone No.: RB02901
• Target: This NeuroD1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 15-45 amino acids from the N-terminal region of human NeuroD1.
• Molecular Weight: 39920 Da
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Disclaimer: For research use only

Alternative Names

Neurogenic differentiation factor 1, NeuroD, NeuroD1, Class A basic helix-loop-helix protein 3, bHLHa3, NEUROD1, BHLHA3, NEUROD

Fluorescent confocal image of HepG2 cell stained with hNeuroD1-Q30. HepG2 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with hNeuroD1-Q30 primary antibody (1:25, 1 h at 37°C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37°C).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37°C). Nuclei were counterstained with DAPI (blue) (10 µg/ml, 10 min).hNeuroD1-Q30 immunoreactivity is localized to vesicles significantly.

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.

Western blot analysis of hNeuroD1-Q30 in HepG2 cell line lysates (35 ug/lane). NEUROD1 (arrow) was detected using the purified Pab.

ES cells were transiently transfected with flag-tagged mouse NeuroD1 (tagged on N-term).Fixed 24h post transfection.Stained for flag tag (red) to check that some cells express protein. Most protein was in nucleus but some was cytoplasmic. Stained with NeuroD1 N-term antibodies at 1:100. NeuroD1 N-term antibody showed strong and clear staining with similar pattern to the flag staining.

Quick Database Links

Gene Symbol

This NeuroD1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 15-45 amino acids from the N-terminal region of human NeuroD1.

UniProt

Q13562

RefSeq (Protein):NP_002491.2