NeuroD1 Antibody (N-term)

• Catalog Number: orb1931825
• Category: Antibodies
• Description: Purified Rabbit Polyclonal Antibody (Pab)
• Target: This NeuroD1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 15-45 amino acids from the N-terminal region of human NeuroD1.
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: Rabbit IgG
• Conjugation: Unconjugated
• Reactivity: Human
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• UniProt ID: Q13562
• MW: 39920 Da
• Tested applications: IF, IHC-P, WB
• Dilution range: IF: 1:10~50, IF: 1:100, WB: 1:1000, IHC-P: 1:50~100
• Antibody Type: Primary Antibody
• Clone Number: RB02901
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: Neurogenic differentiation factor 1, NeuroD, Neuro
• Research Area: Cardiovascular Research, Metabolism Research, Neur
• Note: For research use only
• NCBI: NP_002491.2

Fluorescent confocal image of HepG2 cell stained with hNeuroD1-Q30. HepG2 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with hNeuroD1-Q30 primary antibody (1:25, 1 h at 37°C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37°C).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37°C). Nuclei were counterstained with DAPI (blue) (10 µg/ml, 10 min).hNeuroD1-Q30 immunoreactivity is localized to vesicles significantly.

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.

Western blot analysis of hNeuroD1-Q30 in HepG2 cell line lysates (35 ug/lane). NEUROD1 (arrow) was detected using the purified Pab.

ES cells were transiently transfected with flag-tagged mouse NeuroD1 (tagged on N-term).Fixed 24h post transfection.Stained for flag tag (red) to check that some cells express protein. Most protein was in nucleus but some was cytoplasmic. Stained with NeuroD1 N-term antibodies at 1:100. NeuroD1 N-term antibody showed strong and clear staining with similar pattern to the flag staining.