NCL Antibody (Center E443)

SKU: orb1938027

Description

Affinity Purified Rabbit Polyclonal Antibody (Pab)

Images & Validation

• Tested Applications: IF, IHC-P, WB
• Dilution range: IF: 1:10~50, WB: 1:1000, WB: 1:1000, IHC-P: 1:50~100
• Reactivity: Human

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Clone No.: RB17464
• Target: This NCL antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 428-455 amino acids from the Central region of human NCL.
• Molecular Weight: 76614 Da
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Disclaimer: For research use only

Alternative Names

Nucleolin, Protein C23, NCL

Fluorescent confocal image of Hela cell stained with NCL Antibody (Center E443). Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with NCL primary antibody (1:25, 1 h at 37°C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37°C). Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7 units/ml, 1 h at 37°C). Nuclei were counterstained with DAPI (blue) (10 µg/ml, 10 min). NCL immunoreactivity is localized to nucleus significantly.

NCL Antibody (Center E443) immunohistochemistry analysis in formalin fixed and paraffin embedded human breast tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of NCL Antibody (Center E443) for immunohistochemistry. Clinical relevance has not been evaluated.

NCL Antibody (Center E443) western blot analysis in HepG2 cell line lysates (35 ug/lane). This demonstrates the NCL antibody detected the NCL protein (arrow).

Western blot analysis of NCL (arrow) using rabbit polyclonal NCL Antibody (Center E443). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the NCL gene.

Quick Database Links

Gene Symbol

This NCL antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 428-455 amino acids from the Central region of human NCL.

UniProt

P19338

RefSeq (Protein):NP_005372.2