NAPSA Antibody

SKU: orb1251994

Description

NAPSA Antibody

Research Area

Cell Biology

Images & Validation

• Tested Applications: IF, IHC-P, WB
• Reactivity: Human
• Application Notes:
  Western blot: 1-2 µg/mLImmunofluorescence: 1-2 µg/mLImmunohistochemistry (FFPE): 1-2 µg/mL for 30 min at RT (1)Prediluted format: incubate for 30 min at RT (2)Titering of the Napsin A antibody may be required for optimal performance.1. FFPE testing requires sections to be boiled in pH6 10mM citrate buffer for 10-20 minutes, followed by cooling at RT for 20 minutes, prior to staining.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1, kappa
• Clone No.: NPSNA-1
• Immunogen: A recombinant fragment from the human protein was used as the immunogen for the Napsin A antibody.
• Target: NAPSA
• Purification: Protein G affinity chromatography
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
• Concentration: 0.2 mg/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Napsin-A, 3423-, Aspartyl protease 4, ASP4, Asp 4, Napsin-1, TA01/TA02, NAPSA, NAP1, NAPA

Western blot of 1) K562, 2) 293 and 3) A549 cell lysates using Napsin-A antibody (clone NPSNA-1).

IHC testing of FFPE human lung adenocarcinoma with Napsin-A antibody (clone NPSNA-1).

Quick Database Links

Gene Symbol

NAPSA

UniProt

O96009