Nafamostat mesylate

SKU: orb1941012

Description

Nafamostat mesilate is a synthetic serine protease inhibitor, used as an anticoagulant during hemodialysis.

Research Area

Pharmacology & Drug Discovery

Key Properties

• CAS Number: 82956-11-4
• MW: 539.58
• Purity: >98% (HPLC)
• Formula: C₁₉H₁₇N₅O₂·2CH₄O₃S
• SMILES: CS(O)(=O)=O.CS(O)(=O)=O.NC(N)=NC1=CC=C(C=C1)C(=O)OC1=CC2=C(C=C1)C=C(C=C2)C(N)=N
• Target: Serine Protease
• Solubility: Water: 53 mg/mL (98.22 mM); DMSO: 20 mg/mL (37.06 mM)

Bioactivity

• In Vivo:
  Animal model: A129 mice. Dosage: 10 mg/kg. Administration: Intraperitoneal injection (i.p.). Result: Exhibit delayed lethality and improved survival (40%). Animal model: Balloon injury of the rat carotid wall. Dosage: 0.5 mg/mL, 1 mg/mL, 2 mg/mL (dissolved in saline). Administration: Intraperitoneal injection (i.p.). Result: Showed smaller ratios of the neointima/medial area. Showed positive but reduced immunoreactivity of the cells in the neointimal.
• In Vitro:
  Cell Viability Assay Cell line: MDAPanc-28 cells. Concentration: 80 μg/mL. Incubation time: 24 h, 48 h. Result: Substantially reduced the cell viability of MDAPanc-28 cells at both 24 hours and 48 hours. Cell Invasion Assay Cell line: Inhibited NF-κB DNA-binding activity and the degradation of IκBα in a dose-dependent manner as well as in a time-dependent manner. Inhibited NF-κB DNA-binding activity and the degradation of IκBα in a dose-dependent manner as well as in a time-dependent manner. Panc-1 cells Concentration: 0.1 μM, 1 μM, 10 μM Incubation time: 24 h. Result: Observed significant inhibition in Panc-1-Try clones at concentrations as low as 0.1 mM. Western blot analysis. Cell line: MDAPanc-28 cells. Concentration: 10 μg/mL, 20 μg/mL, 40 μg/mL, 80 μg/mL. Incubation time: 3 h, 8 h, 24 h, 48 h. Result: Inhibited NF-κB DNA-binding activity and the degradation of IκBα in a dose-dependent manner as well as in a time-dependent manner. Inhibited phosphorylation of IκBα in a time-dependent manner.

Storage & Handling

• Storage: Storage temperature: -20°C. Stability: ≥ 2 years
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Coahibitor | FUT 175 | Nafamastat

Quick Database Links

Gene Symbol

Serine Protease