NAM-DH Antibody

SKU: orb750403

Description

NAM-DH antibody

Images & Validation

• Tested Applications: ELISA, IP, WB
• Dilution Range: ELISA: 1:5,000 - 1:20,000, IP: 1:100, WB: 1:500 - 1:5,000
• Reactivity: Pseudomonas sp
• Application Notes:
  Anti-N-Acyl Mannosamine-1-Dehydrogenase has been by western blot and is suitable to be assayed against 1.0 ug of N-Acyl Mannosamine-1-Dehydrogenase [E.coli Recombinant] in a standard ELISA using Peroxidase conjugated Affinity Purified anti-Goat IgG [H&L] (Rabbit) and (ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:5,000 to 1:20,000 of the reconstitution concentration is suggested for this product.

Key Properties

• Antibody Type: Primary Antibody
• Host: Goat
• Clonality: Polyclonal
• Isotype: Antiserum
• Immunogen: N-Acyl Mannosamine-1-Dehydrogenase [Recombinant Sequence from Pseudomonas expressed in E.coli ]
• Target: NAM-DH
• Purity: This product was prepared from monospecific antiserum by a delipidation and defibrination. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-goat serum, purified and partially purified N-Acyl Mannosamine-1-Dehydrogenase [E.coli Recombinant]. Cross reactivity against N-Acyl Mannosamine-1-Dehydrogenase from other sources is unknown.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Form/Appearance: Lyophilized
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Concentration: 90 mg/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

goat anti-N-Acylmannosamine-1-Dehydrogenase Antibody, N-acylmannosamine 1-dehydrogenase, NAM-DH

Western Blot Results of Goat Anti-N-Acylmannosamine-1-Dehydrogenase (Pseudomonas) Antibody. Lane 1: SuperSignal Molecular Weight. Lane 2: N-Acylmannosamine-1-Dehydrogenase. Load: 0.05 µg. Primary Antibody: Goat Anti-N-Acylmannosamine-1-Dehydrogenase at 1:1000 overnight at 2-8°C. Secondary Antibody: HRP Streptavidin (p/n orb348767) at 1:40000 at RT for 30 mins. Blocking: Universal blocking buffer (p/n orb348644) for 30 mins at RT. Predicted/Observed MW: ~27.4kDa/~30kDa.

Quick Database Links

Gene Symbol

NAM-DH

UniProt

P22441

RefSeq:P22441.3