MYL12A Antibody

SKU: orb750488

Description

MYL12A antibody

Research Area

Musculoskeletal & Connective Tissue Research

Images & Validation

• Tested Applications: ELISA, IHC, IP, WB
• Dilution Range: ELISA: 1:5,000 - 1:20,000, IHC: 2.5 µg/ml, IP: 1:100, WB: 1:1,000 - 1:5,000
• Reactivity: Human
• Application Notes:
  Anti-Myosin pS19/pS20 phospho specific polyclonal antibody was tested by ELISA and immunoblotting. Immunoblotting was used to show reactivity with unstimulated and stimulated cardiac myocytes. The antibody was also reactive with the phosphorylated form of the immunizing peptide and minimally reactive with the non-phosphorylated form of the immunizing peptide. Although not tested, this antibody is likely functional by immunohistochemistry and immunoprecipitation.

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Antiserum
• Immunogen: Human Myosin Light Chain phospho peptide corresponding to a region near the amino terminus of the human smooth/non-muscle form of myosin regulatory light chain conjugated to Keyhole Limpet Hemocyanin (KLH).
• Target: MYL12A
• Purity: Anti-Myosin pS19/pS20 antibody is directed against the regulatory light chain of smooth and non-muscle myosin. This antiserum is phosphospecific and detects monophosphorylated and diphosphorylated forms of the protein. Reactivity with non-phosphorylated myosin light chain is less than 1% by ELISA. Cross reactivity is expected with myosin light chain from human and mouse. Reactivity with the protein from other species has not been determined. However, the sequence of the immunogen is nearly identical in mammalian and avian species. BLAST search analysis was used to determine that the smooth and non-muscle forms of myosin regulatory light chain have identical sequences. Cross reactivity is expected.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Form/Appearance: Liquid (sterile filtered)
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Concentration: 70 mg/mL
• Expiration Date: 12 months from date of receipt.
• Dry Ice Shipping: Please note: This product requires shipment on dry ice. A dry ice surcharge will apply.
• Disclaimer: For research use only

Alternative Names

rabbit anti-Myosin pS19/pS20 Antibody, Myosin regulatory light chain 12A, Myosin regulatory light chain MRLC3, Myosin regulatory light chain 2, nonsarcomeric, Myosin RLC, MLC-2B

Western Blot of Rabbit Anti-Myosin pS19/pS20 antibody. Lane 1: HeLa whole cell lysate (p/n orb348668). Lane 2: HeLa whole cell lysate + smooth muscle recombinant phospho protein. Lane 3: HeLa whole cell lysate + regulatory light chain recombinant phospho protein. Load: 10 µg of whole cell lysate + 1.0 µg of recombinant protein. Primary antibody: Mysoin pS19/pS20 antibody at 1.0 µg/ml overnight at 4°C. Secondary antibody: Peroxidase rabbit secondary antibody (p/n orb347654) at 1:40000 for 30 min at RT. Blocking: orb348637 for 30 min at RT. Predicted/Observed size: 20 kDa, 20 kDa for RLC.

Western Blot of Rabbit Anti-Myosin pS19/pS20 antibody. Lane 1: Smooth muscle recombinant non-phospho protein. Lane 2: Smooth muscle recombinant phospho protein. Lane 3: Regulatory light chain recombinant non-phospho protein. Lane 4: Regulatory light chain recombinant phospho protein. Load: 1.0 ug of recombinant non-phospho protein, 5.0 ug of recombinant phospho protein. Primary antibody: Mysoin pS19/pS20 antibody at 1.0 ug/ml overnight at 4°C. Secondary antibody: Peroxidase rabbit secondary antibody at 1:40000 for 30 min at RT. Blocking: orb348637 for 30 min at RT. Predicted/Observed size: 20 kDa, 20 kDa for RLC.

Quick Database Links

Gene Symbol

MYL12A

UniProt

P19105

RefSeq:AAH16372.1