Myosin 1G Antibody

SKU: orb345558

Description

Myosin 1G antibody

Images & Validation

• Tested Applications: ELISA, IP, WB
• Dilution Range: ELISA: 1:10,000 - 1:40,000, IP: 1:100, WB: 1:500 - 1:3,000
• Reactivity: Human
• Application Notes:
  This affinity-purified antibody has been tested for use in ELISA, western blotting and possibly in immunoprecipitation as well. Specific conditions for reactivity should be optimized by the end user. By western blot a band approximately 100 kDa in size corresponding to Myosin 1G protein is expected in the appropriate cell lysate or extract.

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a C-terminal region of human Myosin 1G protein.
• Purity: This affinity-purified antibody is directed against human Myosin 1G protein. The product was affinity purified from monospecific antiserum by immunoaffinity purification. A BLAST analysis was used to suggest cross reactivity with Myosin 1G from human, mouse, chimpanzee and rat based on 100% homology with the immunizing sequence. Cross reactivity with Myosin 1G from other sources has not been determined.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Form/Appearance: Liquid (sterile filtered)
• Buffer/Preservatives: Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Concentration: 1.2 mg/mL
• Expiration Date: 12 months from date of receipt.
• Dry Ice Shipping: Please note: This product requires shipment on dry ice. A dry ice surcharge will apply.
• Disclaimer: For research use only

Alternative Names

rabbit anti-Myosin 1G antibody, Unconventional myosin-Ig, Minor histocompatibility antigen HA-2, mHag HA-2, MYO1G

Western blot using Biorbyt's affinity purified anti-Myosin 1G antibody. Lane 1: Jurkat cell lysates (p/n orb348674) [+]. Lane 2: peripheral blood T cell lysates [+]. Lane 3: human spleen lysates [+]. Lane 4: 300.19 cell lysates [+]. Lane 5: 293 cell lysates (p/n orb348669) [-]. Shows detection of a band ~100kDa in size corresponding to Myosin 1G (arrowhead) in Myosin 1G positive whole cell lysates.

Quick Database Links

UniProt

B0I1T2

RefSeq:239582755