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MUSK Antibody (N-term)

Catalog Number: orb1929012

DispatchUsually dispatched within 5-10 working days
$ 140.00
Catalog Numberorb1929012
CategoryAntibodies
DescriptionPurified Rabbit Polyclonal Antibody (Pab)
Species/HostRabbit
ClonalityPolyclonal
Clone NumberRB01497
Tested applicationsFC, IHC-P, WB
Predicted ReactivityMouse, Rat
ReactivityHuman
IsotypeRabbit IgG
Antibody TypePrimary Antibody
Dilution rangeWB: 1:1000, WB: 1:1000, WB: 1:1000, IHC-P: 1:10~50, FC: 1:10~50
Form/AppearancePurified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
ConjugationUnconjugated
MW97056 Da
TargetThis MUSK antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 35-65 amino acids from the N-terminal region of human MUSK.
UniProt IDO15146
NCBINP_001159752.1, NP_005583.1, NP_001159753.1
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
Alternative namesMuscle, skeletal receptor tyrosine-protein kinase,
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NoteFor research use only
Expiration Date12 months from date of receipt.
MUSK Antibody (N-term)

Western blot analysis of MUSK Antibody (N-term) in Jurkat cell line lysates (35 ug/lane). MUSK (arrow) was detected using the purified Pab.

MUSK Antibody (N-term)

MUSK Antibody (N-term) flow cytometric analysis of CEM cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

MUSK Antibody (N-term)

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.

MUSK Antibody (N-term)

HA-tagged MuSK and GFP constructs were transfected into C2C12 cells. Transfected C2C12 cells were treated with cycloheximide (CHX) and at different times after addition of CHX, amounts of WT and mutant MuSK were analyzed by WB with anti-HA antibody. Alpha-tubulin was used as an internal control and transfection efficiency was verified with anti-GFP antibody.

MUSK Antibody (N-term)

MuSK protein expression in extracts of COS cells after transfection with MuSK mutated and GFP constructs. WB with polyclonal MuSK and monoclonal GFP antibodies showed normal expression of the wild-type MuSK protein (WT), diminished expression of the GA mutant MuSK and no expression of the insC mutant or the pcDNA3 vector alone in transfected COS cells. GFP cotransfection was used to verify transfection efficiency.

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    IHC-P,  WB

    Human

    Rabbit

    Polyclonal

    Unconjugated

    100 μl
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    FC,  IHC,  WB

    Human

    Rabbit

    Polyclonal

    Unconjugated

    100 μl