Mucin-3 Antibody

SKU: orb2638917

Description

It recognizes a protein of HMW, identified as mucin 3 glycoprotein (MUC3). Its epitope localizes between amino acids SITTTE. This mAb shows no cross-reaction with human milk fat globule membranes, MUC1, or MUC2. MUC3 is distributed in colon and rectum, and is also present to a lesser extent in breast, lung and salivary gland tissues. The Mucins are a family of highly glycosylated, secreted proteins with a basic structure consisting of a variable number of tandem repeats (VNTRs) encoded by 60 base pairs (Mucin 1), 69 base pairs (Mucin 2) and 51 base pairs (Mucin 3). The number of repeats is highly polymorphic and varies among different alleles. Mucin 1 proteins are expressed as type I membrane proteins in addition to secreted forms. Mucin 1 is aberrantly expressed in epithelial tumors including breast carcinomas. Mucin 2 coats the epithelia of the intestines and airways and is associated with colonic tumors. Mucin 3 is a major component of various mucus gels and is broadly expressed in normal and tumor cells.

Images & Validation

• Tested Applications: IHC-P
• Dilution range: The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
• Reactivity: Human
• Application Notes:
  Optimal dilution of the Mucin-3 antibody should be determined by the researcher.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: Mouse IgG2a, kappa
• Clone No.: M3.1
• Immunogen: Amino acids (C-HSTPSFTSSITTTETTSHSTPSFTSSITTTETTS) which contains two of the MUC3 tandem repeats was used as the immunogen for the Mucin-3 antibody.
• Purification: Protein G affinity chromatography
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Disclaimer: For research use only

IHC: Formalin-fixed, paraffin-embedded human colon carcinoma stained with Mucin-3 antibody (M3.1).

IHC: Formalin-fixed, paraffin-embedded human gastric carcinoma stained with Mucin-3 antibody (M3.1).

IHC: Formalin-fixed, paraffin-embedded human gastric carcinoma stained with Mucin-3 antibody (M3.1).

SDS-PAGE Analysis of Purified, BSA-Free Mucin-3 Antibody (clone M3.1). Confirmation of Integrity and Purity of the Antibody.

Quick Database Links

UniProt

Q02505