HAVCR2/TIM-3 Rabbit Polyclonal Antibody

• Catalog Number: orb186446
• Category: Antibodies
• Description: HAVCR2/TIM-3 Rabbit Polyclonal Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, WB
• Predicted Reactivity: Rat
• Reactivity: Human, Mouse
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from mouse HAVCR2 (151-250/281aa)
• Antibody Type: Primary Antibody
• Concentration: 1mg/ml
• Dilution range: WB=1:500-2000, ELISA=1:5000-10000
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 29 kDa
• Target: HAVCR2
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Alternative names: Hepatitis A virus cellular receptor 2 homolog; CD3
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Sample: Liver (Rat) Lysate at 40 ug, Primary: Anti-TIM 3 (orb186446) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 29 kD, Observed band size: 49 kD.

Sample: Spleen (Mouse) Lysate at 40 ug, Primary: Anti-TIM 3 (orb186446) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 29 kD, Observed band size: 49 kD.

Sample: Thymus (Mouse) Lysate at 40 ug, Raw264.7 (Mouse) Cell Lysate at 30 ug, Primary: Anti-TIM 3 (orb186446) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 29 kD, Observed band size: 49 kD.

Tissue/Cell: rat liver tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-TIM-3 Polyclonal Antibody, Unconjugated (orb186446) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.