HAVCR2/TIM-3 Rabbit Polyclonal Antibody

SKU: orb186446

Description

HAVCR2/TIM-3 Rabbit Polyclonal Antibody

Images & Validation

• Tested Applications: ELISA, WB
• Dilution range: WB=1:500-2000, ELISA=1:5000-10000
• Reactivity: Mouse
• Predicted Reactivity: Human

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Recombinant human TIM-3 protein (22-200/301aa)
• Target: HAVCR2
• Molecular Weight: 29 kDa
• Purification: Affinity purified by Protein A

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Disclaimer: For research use only

Alternative Names

Hepatitis A virus cellular receptor 2 homolog; CD366; CD 366; TIM-3; HAVR2_MOUSE; Kidney injury molecule 3; KIM 3; KIM3; T cell immunoglobulin and mucin domain containing 3; T cell immunoglobulin mucin 3; T-cell immunoglobulin and mucin domain-containing protein 3; T-cell immunoglobulin mucin receptor 3; T-cell membrane protein 3; TIM3; TIMD-3; TIM 3; TIMD3.

Sample: Liver (Rat) Lysate at 40 ug, Primary: Anti-TIM 3 (orb186446) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 29 kD, Observed band size: 49 kD.

Sample: Spleen (Mouse) Lysate at 40 ug, Primary: Anti-TIM 3 (orb186446) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 29 kD, Observed band size: 49 kD.

Sample: Thymus (Mouse) Lysate at 40 ug, Raw264.7 (Mouse) Cell Lysate at 30 ug, Primary: Anti-TIM 3 (orb186446) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 29 kD, Observed band size: 49 kD.

Tissue/Cell: rat liver tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-TIM-3 Polyclonal Antibody, Unconjugated (orb186446) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.

Quick Database Links

Gene Symbol

HAVCR2

UniProt

Q8TDQ0