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| Catalog Number | orb1533674 |
|---|---|
| Category | Antibodies |
| Description | MRGPRF Antibody |
| Target | MRGPRF |
| Clonality | Polyclonal |
| Species/Host | Rabbit |
| Conjugation | Unconjugated |
| Reactivity | Human |
| Concentration | 1 mg/ml |
| Buffer/Preservatives | PBS, 0.1% Sodium Azide. PBS, 0.1% Sodium Azide |
| Purification | Immunoaffinity purified |
| Immunogen | Synthetic 18 amino acid peptide from N-Terminus of human MRGPRF. Percent identity with other species by BLAST analysis: Human, Gorilla, Marmoset, Dog, Bovine, Horse (94%); Mouse, Rat, Hamster, Elephant, Panda, Pig (88%). |
| Tested applications | ELISA, IHC, IHC-P |
| Dilution range | ELISA, IHC, IHC-P (10 µg/ml) |
| Application notes | Further information: Immunohistochemistry: The unconjugated form was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after proteinase K antigen retrieval. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for the unconjugated form was determined to be 10 ug/ml. |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Alternative names | MRGPRF, G protein-coupled receptor 168, G-protein Read more... |
| Note | For research use only |
ELISA, IF, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
IHC, IHC-P | |
Bovine, Canine, Equine, Hamster, Human, Monkey, Mouse, Porcine, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
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