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Item 1 of 3
MOX1 (Meox1) Antibody (N-term)
Catalog Number: orb1929413
| Catalog Number | orb1929413 |
|---|---|
| Category | Antibodies |
| Description | Affinity Purified Rabbit Polyclonal Antibody (Pab) |
| Target | This MOX1 (Meox1) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 7-38 amino acids from the N-terminal region of human MOX1 (Meox1). |
| Clonality | Polyclonal |
| Species/Host | Rabbit |
| Isotype | Rabbit IgG |
| Conjugation | Unconjugated |
| Reactivity | Human |
| Form/Appearance | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| UniProt ID | P50221 |
| MW | 27997 Da |
| Tested applications | IF, WB |
| Dilution range | IF: 1:10~50, WB: 1:1000, WB: 1:1000 |
| Clone Number | RB10382 |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
| Alternative names | Homeobox protein MOX-1, Mesenchyme homeobox 1, MEO Read more... |
| Note | For research use only |
| NCBI | NP_054705.1, NP_004518.1, NP_001035091.1 |
Fluorescent confocal image of Hela cell stained with Meox1 (Human N-term).Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with Meox1 primary antibody (1:25, 1 h at 37°C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37°C).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37°C). Nuclei were counterstained with DAPI (blue) (10 µg/ml, 10 min). Meox1 immunoreactivity is localized to Nucleolus significantly.
Western blot analysis of anti-Meox1 Antibody (N-term) in 293 cell line lysates (35 ug/lane). Meox1 (arrow) was detected using the purified Pab.
Anti-Meox1 (Human N-term) at 1:1000 dilution + human heart lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 28 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
