MOX1 (Meox1) Antibody (N-term)

• Catalog Number: orb1929413
• Category: Antibodies
• Description: Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Target: This MOX1 (Meox1) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 7-38 amino acids from the N-terminal region of human MOX1 (Meox1).
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: Rabbit IgG
• Conjugation: Unconjugated
• Reactivity: Human
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• UniProt ID: P50221
• MW: 27997 Da
• Tested applications: IF, WB
• Dilution range: IF: 1:10~50, WB: 1:1000, WB: 1:1000
• Clone Number: RB10382
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: Homeobox protein MOX-1, Mesenchyme homeobox 1, MEO
• Research Area: Cell Biology, Signal Transduction, Stem Cell & Dev
• Note: For research use only
• NCBI: NP_004518.1, NP_054705.1, NP_001035091.1
• Expiration Date: 12 months from date of receipt.

Fluorescent confocal image of Hela cell stained with Meox1 (Human N-term).Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with Meox1 primary antibody (1:25, 1 h at 37°C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37°C).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37°C). Nuclei were counterstained with DAPI (blue) (10 µg/ml, 10 min). Meox1 immunoreactivity is localized to Nucleolus significantly.

Western blot analysis of anti-Meox1 Antibody (N-term) in 293 cell line lysates (35 ug/lane). Meox1 (arrow) was detected using the purified Pab.

Anti-Meox1 (Human N-term) at 1:1000 dilution + human heart lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 28 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.