Mouse IgG2b isotype control Peroxidase Antibody

SKU: orb343786

Description

Mouse IgG2b isotype control Peroxidase Antibody

Research Area

Protein Biochemistry

Images & Validation

• Tested Applications: FC, IF
• Dilution Range: FC: 1:1000-1:5000, IF: 1:1000-1:5000
• Application Notes:
  Mouse IgG2b isotype control can be utilized as a control or standard reagent in Flow cytometry, Western Blotting, and ELISA experiments where determination of sample isotype is important.

Key Properties

• Isotype: IgG2b
• Clone No.: MB2
• Purity: This product has been prepared from immunodeficient mouse ascites by protein A chromatography using specific conditions for subclass purification. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Mouse IgG2b and anti-Mouse serum. Isotyping assay resulted the material is non-reactive with antisera to mouse IgG1, IgG2a, IgG3, IgM, and IgA. Peroxidase Conjugated Mouse IgG2b was proven by Dot Blot.
• Conjugation: HRP

Storage & Handling

• Storage: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Form/Appearance: Lyophilized
• Buffer/Preservatives: Preservative: 0.01% (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!. Stabilizer: 10 mg/mL Bovine Serum Albumin (rAlbumin) - Immunoglobulin and Protease free; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Concentration: 1.0 mg/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Peroxidase conjugated Mouse IgG2b isotype control, Mouse IgG2b subclass isotype control HRP conjugation