Mouse IgG2b (Gamma 2b chain) Antibody Fluorescein Conjugated

SKU: orb347542

Description

Mouse IgG2b (Gamma 2b chain) antibody (FITC)

Images & Validation

• Tested Applications: FC, FLISA, IF, WB
• Dilution Range: FLISA: 1:10,000 - 1:50,000, FC: 1:500 - 1:2,500, IF: 1:1,000 - 1:5,000
• Reactivity: Mouse
• Application Notes:
  Anti-Mouse IgG2b Fluorescein Antibody has been tested by western blot and is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.

Key Properties

• Antibody Type: Secondary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Mouse IgG2b heavy chain
• Purity: This product was prepared from monospecific antiserum by immunoaffinity chromatography using antigens coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein, anti-Rabbit Serum and Mouse Serum. Specificity was confirmed by ELISA.
• Conjugation: FITC

Storage & Handling

• Storage: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Form/Appearance: Lyophilized
• Buffer/Preservatives: Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: 10 mg/mL Bovine Serum Albumin (rAlbumin) - Immunoglobulin and Protease free; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Concentration: 1.0 mg/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Rabbit Anti-Mouse IgG2b (gamma 2b chain) Antibody fluorescein Conjugated, Rabbit Anti-Mouse IgG2b Antibody FITC Conjugation

Western Blot of Rabbit anti-Mouse IgG2b (Gamma 2b chain) Fluorescein Conjugated Antibody. Lane 1: Mouse IgG2b. Lane 2: None. Load: 50 ng per lane. Primary antibody: None. Secondary antibody: Fluorescein rabbit secondary antibody at 1:1000 for 60 min at RT. Block: orb348637 for 30 min at RT. Predicted/Observed size: 55 kDa, 55 kDa for Mouse IgG2b. Other band(s): Light Chain.