Mouse IgG2a isotype control Phycoerythrin Antibody

SKU: orb343793

Description

Mouse IgG2a isotype control Phycoerythrin Antibody

Research Area

Protein Biochemistry

Images & Validation

• Tested Applications: DOT, FC, IF
• Dilution Range: FC: 1:1000-1:5000, IF: 1:1000-1:5000
• Application Notes:
  Mouse IgG2a isotype control has been tested in dot blot and can be utilized as a control or standard reagent in Flow cytometry, Western Blotting, and ELISA experiments where determination of sample isotype is important.

Key Properties

• Isotype: IgG2a
• Clone No.: M2A
• Purity: This product was prepared from ascitic fluid by affinity chromatography. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Mouse IgG2a and anti-Mouse Serum. Isotyping assay resulted the material is non-reactive with antisera to mouse IgG1, IgG2b, IgG3, IgM, and IgA. Phycoerythrin Conjugated Mouse IgG2a was proven by Dot Blot.
• Conjugation: RPE

Storage & Handling

• Storage: Store vial at 4° C prior to restoration. Restore with deionized water (or equivalent). This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Centrifuge product if not completely clear after standing at room temperature. Do not freeze after reconstitution. Store reagent in the dark. Use subdued lighting during handling and incubation of cells prior to analysis.
• Form/Appearance: Lyophilized
• Buffer/Preservatives: Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: 10 mg/mL Bovine Serum Albumin (rAlbumin) - Immunoglobulin and Protease free; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Concentration: 1.0 mg/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Phycoerythrin conjugated Mouse IgG2a isotype control, Mouse IgG2a subclass isotype control PE conjugation

Dot Blot results of Mouse IgG2a R-Phycoerythrin conjugate. Dots are Ms IgG2a RPE at 100 ng, 33.3 ng, 11.1 ng, 3.70 ng, 1.23 ng levels. Blocking: orb348637 for 60 min at RT. Primary Antibody: none. Secondary Antibody: none.