Mouse IgG2a isotype control Fluorescein Antibody

SKU: orb343781

Description

Mouse IgG2a isotype control Fluorescein Antibody

Research Area

Protein Biochemistry

Images & Validation

• Tested Applications: DOT, FC
• Dilution Range: FC: 1:1000-1:5000
• Application Notes:
  Mouse IgG2a isotype control has been tested in dot blot and can be utilized as a control or standard reagent in Flow cytometry, Western Blotting, Immunofluorescence, and ELISA experiments where determination of sample isotype is important.

Key Properties

• Isotype: IgG2a
• Clone No.: M2A
• Purity: This product has been prepared from normal mouse serum by immunoaffinity chromatography using antibodies coupled to agarose beads. Extensive cross-adsorbtion was performed to remove any unwanted subclasses. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Mouse IgG2a and anti-Mouse Serum. Isotyping assay resulted the material is non-reactive with antisera to mouse IgG1, IgG2b, IgG3, IgM, and IgA. Fluorescein Conjugated Mouse IgG2a was proven by Dot Blot.
• Conjugation: FITC

Storage & Handling

• Storage: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Form/Appearance: Lyophilized
• Buffer/Preservatives: Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: 10 mg/mL Bovine Serum Albumin (rAlbumin) - Immunoglobulin and Protease free; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Concentration: 1.0 mg/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Fluorescein conjugated MOUSE IgG2a subclass isotype control, MOUSE IgG2a isotype control FITC conjugation

Dot Blot of Mouse IgG2a Isotype Control Fluorescein Conjugated. Ms IgG2a Isotype Control FITC Conjugate (1) 100 ng, (2) 33.33 ng, (3) 11.11 ng, (4) 3.70 ng, (5) 1.23 ng. Block: Fluorescent Buffer (p/n orb348637) RT for 30 mins.