Mouse IgG1 Secondary Antibody Peroxidase Conjugated

• Catalog Number: orb347554
• Category: Antibodies
• Description: Mouse IgG1 antibody (Peroxidase)
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: IgG
• Conjugation: HRP
• Reactivity: Mouse
• Form/Appearance: Lyophilized
• Concentration: 1.0 mg/mL
• Buffer/Preservatives: 0.01% (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!
• Purity: This product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum, Mouse IgG and Mouse Serum. Specificity was confirmed by ELISA against other mouse or human heavy chain isotypes. Cross reactivity with Mouse IgG2b and IgG3 is 1%.
• Immunogen: Mouse IgG1 heavy chain
• Tested applications: ELISA, IHC, WB
• Dilution range: ELISA: 1:20,000 - 1:200,000, IHC: 1:500 - 1:2,500, WB: 1:2,000 - 1:20,000
• Application notes: Mouse IgG1 secondary antibody conjugated to HRP is available in a variety of formats. Anti-Mouse IgG1 Peroxidase Antibody has been tested by ELISA and western blot and is suitable for ELISA, Immunohistochemistry western blotting as well as other anti IgG1 antibody based assays.
• Antibody Type: Secondary Antibody
• Storage: Store secondary antibody at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Alternative names: Rabbit Anti-Mouse IgG1 (Gamma 1 chain) Antibody pe
• Note: For research use only

Western blot of Peroxidase conjugated Rabbit Anti-Mouse IgG1 (Gamma 1 chain) secondary antibody. Lane 1: Mouse IgG1. Lane 2: None. Load: 50 ng per lane. Primary antibody: None. Secondary antibody: Peroxidase rabbit secondary antibody at 1:1000 for 60 min at RT. Blocking: orb348637 for 30 min at RT. Predicted/Observed size: 55 kDa, 55 kDa for Mouse IgG1 (Gamma 1 chain). Other band(s): None.